?????? ?????????,???????,????,??,????,??????800????,??60??????????????ο?
????????????????????????????????У????á????ɡ??????????????????????????????????????????????????????????????

miRNA??????????????????????????????

?????????????? ??????2019-07-11

???????? ???????????????????PCOS???????举???????????????????????????????????????????????????????????????????????????????MiRNA???СRNA???????????и??????????????п?????????????????????????????????????????????????????????????????miRNA???????????????????????????????????????????????????????????????????????????????????????о???????miRNA????????????????????????????????????????PCOS??????????

?????????????RNA; ???????; ?????????; ????????????;

????Abstract????Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disease in women of childbearing age??follicular developmental maturation is its main clinical feature and is generally thought to be caused by environmental and genetic inheritance.MiRNA, is highly conserved in evolution and spatially specific in expression, and exerts its regulatory function at the post-transcriptional level,regulating cell proliferation, differentiation, apoptosis and other activities.Because mirnas are widely expressed in female reproductive organs such as uterus and bilateral appendages, their abnormal expressions will directly affect the physiological function of ovary, normal development of follicles and oocytes.A large number of studies have shown that miRNA promotes the generation and development of PCOS in granulosa cell proliferation, insulin resistance, hyperandrogenemia and other aspects.

????Keyword????mirna; Insulin resistance; Hyperandrogenemia; Polycystic ovary syndrome;

??????????????????PCOS???????举???????????????????????????й?10??????е???????в?????飬19??45????й????帾??иò?????????5.6%[1]??PCOS???????????????????????????????????????????????????????????????????????????????????????????????л??????????????????????????????????????????????????????????????????????????[[2]][[3]][[4]]??????????????????????????????????о?????miRNA ??PCOS??????????????????????????miRNA???????PCOS?????????????????????????PCOS??????е??????miRNA??PCOS???????????????????????????????о????????????ο???

????1.? MiRNA?????

????MiRNA?????Dicer???????????23???????????е?????????????????RNA?????????????????У??????λ??5???? 2~8 ???????seed?????????????mRNA??3??UTR(3-untranslation region,3?????????)???????????????????mRNA???????????????б????????????????????????????????????????????????????????????а???????????[5]????????????miRNA???????????????????????1527??????miRNA???????????30?????????[2,5]??

????2.? MiRNA???????????

??????????????????????????????????????????????????????????????????乲??????????????鵼??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????[6]??
?

miRNA??????????????????????????????
?

????2.1??MiRNA???????????????

????Xu??[7]???miRNAо????????PCR??????????PCOS????????????miRNA????????????е????????????????59??miRNA???У?PCOS??????21??miRNA????????38??????μ??????????????miRNA??????PCOS???????????????????miRNA?????????Notch????·??PI3K/AKT?·??Wnt?·?й??Xue??[8]??????????о????????7??miRNA?????????г????????7?????????????μ????????qRT-PCR??????miRNA-3188??miRNA-3135b????????????????FSH???????????miRNA-3188?????????????????????о???????miRNA?????????????PCOS????????????????

????2.2?????MiRNA?????????????

????DAPK1??????????????1????????caspase?\3???????????????????·?е????????????????????????????з??????????????????JAK2/STAT3·(Janus kinase 2/signal ers and activators of protein 3)??????????????????????·Э???????????[9]??????DAPK1???????PI3K/Akt ??ERK????·????????????????????[10]??PCOS???????????и????miRNA-141-3p??????л???DAPK1??3??-UTRλ??,????DAPK1 mRNA???????????????????????????[11]??

??????????????O1 ??factor forkhead box O1??FOXO1????miRNA-183~96~182??л??????????????????????????????????????????siRNA??С????RNA????????ó???????????????е??????????????????????PCOS???????????и????miRNA-183~96~182??????????FOXO1 mRNA??????????????????????????????S????????????????????????????[12]??miR-183~96~182????????????????????????????????????????????????????б???[13]??

????Notch????·??????????????????????????????????Notch??????????????????????????????????????????????????????????????????????????MAPK/ERK???????????????/???????????????????·Э????????????????й?????????????????????????????????????[14]??Notch 3??MAPK 3??mRNA?????????miRNA-483-5p???????????????и????miRNA-483-5p?????????????3??UTR?????????????????????????????????[15]??

???????????miRNA??????????????????????????miRNA????????????????·??miRNA-145???????????????????????????????????????????????θ???????????????????????б???????μ?[4][16]??Cai??[17]?о????????PCOS???????????е????miRNA-145?????????????????????????????????????????IRS1?й???????о????????????????????????????miRNA-145???????IRS1??????????????????????Zhong??[18]??????PCOS?????б???μ??? miR-19b?????????????IGF-1(???????????????)???????????????????D1??CDK1?????????????????????????????????????????????????miR-19b???????????????????Geng[19]???????????????????miR-99a??????????????????80 ng/ml???PCOS????????miR-99a????л???IGF-1R ??????????????????Σ?????????????????????????????miRNA-145??miR-19b??miR-99a???????????????????????????????????????????????????γ?????????????Jiang??[20]?о????????miR-324-3p??PCOS????????е??????????????????????WNT2B???????????????

????????????miRNA?????????е??????????????????????????????????????????????????γ??????????о????μ??????

????3.? MiRNAs?????????

??????????????????????????????????????????Ч???????????????????IR???????????????????????????????????????????????γ???????????IH????????????????????????????????????????????????????????????·??P450????????????????й??????????????/????????????????????????[[21]][[22]]??

????3.1??MiRNA???GLUT4????

????GLUT4??glucose transporter 4??????????????4???????????????????鵼????????????????????????????????AT?????л??????????????????GLUT4????????????????????GLUT4??????????沢????????????????????????????????GLUT4????????????????????????????????????棬????????????????????????????????????????????????????????????????????????????2??????????????????????????????????????[23]??Yang??[24]???????PCR???????????????????????PCOSС??????У?miRNA-33b-5p?????????????????????????????????????????????GLUT4?????????????HMGA2?????????鵰??2????????????GLUT4??5???????????????????????????SREBF1??????????????????1??????????GLUT4????????miRNA-33b-5p?????????HMGA2????????GLUT4?????????????????????????????????????miRNA-33b-5p?????????????????????????????HMGA2??SREBF1??GLUT4 mRNA????????????????????????????Xiao??[25]??RNA????????????з?????????miRNA-93??GLUT4 3??UTR?????λ??????????????GLUT4????????????GLUT4??????

????3.2??MiRNA????????????????

????MiRNA??????????GLUT4????????????????????????????????????????????????????????????????P85???????????????-3-?????Phosphatidylinositol-4,5-bisphosphate 3-kinase??PI3K???????????????????????·?з?????????á?Liu??[26]???????PCOS??????miRNA-29???壨a??b??c????????μ??????????????AICAR??5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside??????????????AMP-activatedproteinkinase(AMPK)????????????μ?miRNA-29???????????л???P85????????????????????????·?????????????????????????????????1(Insulin-like growth factor 1 ??IGF-1??????????????????????????????????????IGF-1R????????坝??????????????????????????Ч?????????????????????????????????????Li??[27]??鷢??????miR-122???????miR-122?????????????IGF-1 mRNA??3'UTR?????μ???IGF-1????miR-122?????IGF-1 3'UTRλ??????????????Ч??????????????miR-122?????IGF-1??????????????????IGF1????????????????????????????????IGF-1??????????????????????????????????????????????????????[28]??????miR-122????????????????????????????????????????????鼰??????????????????????黼?????????????PCOS?????????miR-320??????????miRNA-320???IRS?????????????????????????1/2??extracellular regulated protein kinases,ERK1/2???·????????????IR????????????[29]?????????ì??????AS??[30]???о????miR-320??PCOS????????????????е??????????????????????PCOS??????????????????????й??????miR-320??PCOS?????е?????????????IR?????л????????????????

?????????miRNA????????GLUT4???????????????????????????????????????PCOS???????????????????????????

????4.? MiRNA??????????

??????????У?????????????????????????????????????У????????????????????????????????????????????????????????????????????????????????????????????????????[31]??

????4.1??MiRNA?????????????

??????????????/????????????????壨LHCGR??????G??????????峬???壬????????????????????????????????У????????????????????????,?????LH??????LH???LHCGR???????á???????????????????????????????????????????????P450-17α???cytochrome P450 17α-hydroxylase??CYP17????3β-HSD(3β-hydroxysteroid dehydrogenase,3β-????????????)??????????CYP11A??CYP17??????????????????????????????????????????3β-HSD??????????????????????????????????壬??????????????????CYP19????????????????(aromatase, CYP19)????????P450???е????, ???????????????????19λ????A????????, ????γ?????????, ?????????????????????????????creb1?? cyclic AMP response element (CRE)-binding protein 1????????Wang??[32]?????PCOS?????????????miRNA-27a-3p????????????????creb1????????Cyp19a1??????????????????????????????????????

????????IGF1??????????GnRH?????????Gn??????????Э?LH????????????????????????????????IGF2??Э?FSH??????????????????????????????????????????????????????[33]??PCOSС??????????miRNA-186???????IGF1?????????IGF2???[34]?????IGF1???????????????????ɡ?IGF2??????????????????????????????????????????????????????PCOS??????????

????4.2??MiRNA?????????????????????????

????????????????????????????PCOS???????????????????????????????????????顣??Xue??[35]??????У?PCOS?????е????miRNA-92a????????????????????CYP17????????????????????IRS-2???????????????????????????????????????????????·????????棬????????????д???????о???

?????????miRNA???????????????????????????????????????????????????????????????????????????????????????????????????

????5.MiRNA?????PCOS??????????

????????????????????????????????????????????????????????????miRNA????PCOS???????????????????????????????????????????????????????????????????????????????????????miRNA????????????????????δ?????

??????????PCOS????????????n=12????11????????????????6??????????????????????о?????????????6??miRNA??PCOS?????б??????????????н????????????????????????????????????????2??miRNA??microRNA-153??140-5p?????????????miRNA????????????????????????????????????????????[36]??Song??[37]???21??PCOS????????????????о???????????????????????????????к?????о???????з???PCOS??????????miRNA-4522??miRNA-324-3p??miRNA-6767-5p????μ?С??0.67??????????qRT-PCR??????????miRNA-6767-5p??miRNA-4522??PCOS?????????????????????????????????????????????????????????GO??????????????????????????????????????????????????????????????????????????????????????????????????????PCOS?????????????顣

???????????????????????????miRNA?????PCOS??????μ??????????????????е?1????????2018???л??????????????????????????????????????[38]???????????????????????????????????????????????????????????????о??????ü???????

????6.????????

?????????????о??????miRNA???????????PCOS????????????????????????????????????????????????????е????????????????о??????????miRNA????????PCOS??????????????miRNA?????????mRNA???????mRNA 3'UTR??????????miRNA?????????????????????????????????????о??????С????????????????????????棬????????ì????о??????????????miRNA??????????????о??????????????????????PCOS?????miRNA????????????????????????????????????miRNA???????μ?PCOS??????????????????????????PCOS????????????????????????????????μ??·??

?????ο?????

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